High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer

Aleksandra Ciarka; Michał Kunc; Marta Popęda; Aleksandra Łacko; Barbara Radecka; Marcin Braun; Joanna Pikiel; Maria Litwiniuk; Katarzyna Pogoda; Ewa Iżycka-Świeszewska; Anna Zeller; Magdalena Niemira; Rafał Pęksa; Wojciech Biernat; Elżbieta Senkus

doi:10.5114/wo.2024.139375

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

Current issue Archive Manuscripts accepted About the journal Supplements Addendum Special Issues Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

1/2024
vol. 28

Send email

Copy url:

abstract:

Original paper

High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer

Aleksandra Ciarka

¹

,

Michał Kunc

¹

,

Marta Popęda

¹

,

Aleksandra Łacko

^{2, 3}

,

Barbara Radecka

^{4, 5}

,

Marcin Braun

⁶

,

Joanna Pikiel

⁷

,

Maria Litwiniuk

⁸

,

Katarzyna Pogoda

⁹

,

Ewa Iżycka-Świeszewska

¹⁰

,

Anna Zeller

¹¹

,

Magdalena Niemira

¹¹

,

Rafał Pęksa

¹

,

Wojciech Biernat

¹

,

Elżbieta Senkus

¹²

Department of Pathology, Medical University of Gdańsk, Gdańsk, Poland
Department of Oncology, Wrocław Medical University, Wrocław, Poland
Department of Oncology, Breast Unit, Lower Silesian Oncology Centre, Wroclaw, Poland
Department of Oncology, Institute of Medical Sciences, University of Opole, Opole, Poland
Department of Clinical Oncology, Tadeusz Koszarowski Cancer Centre in Opole, Opole, Poland
Department of Pathology, Chair of Oncology, Medical University of Łódź, Łódź, Poland
Department of Oncology, Morski Hospital, Gdynia, Poland
Department of Clinical Oncology, Greater Poland Cancer Centre, Poznań, Poland
Department of Breast Cancer and Reconstructive Surgery, Maria Sklodowska Curie National Research Institute of Oncology, Warsaw, Poland
Department of Pathology and Neuropathology, Medical University of Gdańsk, Gdańsk, Poland
Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk, Gdańsk, Poland
Department of Oncology and Radiotherapy, Medical University of Gdańsk, Gdańsk, Poland

Contemp Oncol (Pozn) 2024; 28 (1): 75–83

DOI: https://doi.org/10.5114/wo.2024.139375

Online publish date: 2024/05/03

View full text Get citation

EndNote

Papers, Reference Manager, RefWorks, Zotero

AMA

Ciarka A, Kunc M, Popęda M, et al. High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer. Contemporary Oncology/Współczesna Onkologia. 2024;28(1):75-83. doi:10.5114/wo.2024.139375.

APA

Ciarka, A., Kunc, M., Popęda, M., Łacko, A., Radecka, B.,  & Braun, M. et al. (2024). High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer. Contemporary Oncology/Współczesna Onkologia, 28(1), 75-83. https://doi.org/10.5114/wo.2024.139375

Chicago

Ciarka, Aleksandra, Michał Kunc, Marta Popęda, Aleksandra Łacko, Barbara Radecka, Marcin Braun,  and Joanna Pikiel et al. 2024. "High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer". Contemporary Oncology/Współczesna Onkologia 28 (1): 75-83. doi:10.5114/wo.2024.139375.

Harvard

Ciarka, A., Kunc, M., Popęda, M., Łacko, A., Radecka, B., Braun, M., Pikiel, J., Litwiniuk, M., Pogoda, K., Iżycka-Świeszewska, E., Zeller, A., Niemira, M., Pęksa, R., Biernat, W.,  and Senkus, E. (2024). High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer. Contemporary Oncology/Współczesna Onkologia, 28(1), pp.75-83. https://doi.org/10.5114/wo.2024.139375

MLA

Ciarka, Aleksandra et al. "High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer." Contemporary Oncology/Współczesna Onkologia, vol. 28, no. 1, 2024, pp. 75-83. doi:10.5114/wo.2024.139375.

Vancouver

Ciarka A, Kunc M, Popęda M, Łacko A, Radecka B, Braun M et al. High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer. Contemporary Oncology/Współczesna Onkologia. 2024;28(1):75-83. doi:10.5114/wo.2024.139375.

PlumX metrics:

Introduction:
This study aimed to evaluate the impact of tumour-infiltrating lymphocytes (TILs) on the expression of immune-related genes and prognosis in single hormone receptor-positive breast cancer.

Material and methods:
Tumour-infiltrating lymphocytes were analysed according to the guidelines of the International TILs Working Group in a cohort of 206 patients with single hormone receptor-positive breast cancer. Of these, 44.7% were classified as ER+/PgR–/HER2–, 18.4% as ER+/PgR–/HER2+, 26.2% as ER–/PgR+/HER2–, and 10.7% as ER–/PgR+/HER2+. Moreover, in 52 samples the analysis of gene expression profiling was performed using nCounter technology.

Results:
Most cases (74.3%) showed at least 1% of stromal TILs, with a median of 4%, mean of 16.3%, and interquartile range of 0–20%. ER–/PgR+ tumours displayed significantly higher TILs density than ER+/PgR– cases (p < 0.001, Wilcoxon test), regardless of HER2 status. The abundance of TILs was positively associated with ER–/PgR+ phenotype, higher Ki-67, and higher grade, but not with age, tumour size, or regional and distant metastases at diagnosis. Additionally, in ER+/PgR– subgroup higher TILs were associated with HER2-positive status. Stromal TILs > 5% were associated with better survival in the whole group, but this effect was less prominent in ER–/PgR+ patients. We identified 50 differentially expressed genes (DEGs) between single hormone receptor-positive breast tumours with high and low TILs, including 39 up-regulated and 11 down-regulated genes in the high TILs group.

Conclusions:
The up-regulated expression of immune-related genes was consistent also among separately analysed single hormone receptor-positive groups (ER+/PgR– and ER–/PgR+).

keywords:

High tumour-infiltrating lymphocytes correlate with distinct gene expression profile and favourable survival in single hormone receptor-positive breast cancer

gene expression, tumour infiltrating lymphocytes, prognosis, breast cancer